Barcode of Life: Guelph-based DNA database a digital Noah's ark aiming to ID all living organisms
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DNA databank at the University of Guelph campus
is now the largest genetic reference library in the world
It is the main hub of the global International Barcode of Life project, a digital Noah’s Ark, helping scientists discover an unexpected treasure trove of new species in the wild, and has also emerged as a watchdog of sorts on food products.
Guelph’s genetic database, which currently includes 240,000 species stored on dedicated computer servers linked to the Internet, aims to identify all living organisms on Earth.
“The goal is for any person to recognize any life form,” said Paul Hebert, biology professor at the University of Guelph, and founder of DNA barcoding.
What started out in one standard-issue university lab a decade ago has expanded to two new buildings on the southern edge of the Guelph campus. Rooms are filled with lab-coated researchers and students working on state-of-the-art equipment such as fully automated robotic machines that hum along processing almost 100 samples at once, as opposed to the old-fashioned manual method of one sample at a time.
At the Guelph lab, areas are set aside for extracting the DNA through chemical processes from tissue samples sent to them from all corners of the world.
A standardized part of the DNA genome is replicated millions of times in a desktop machine called an amplifier. The individual units of DNA are then read in a larger machine called a sequencer that downloads to a computer the order of appearance of the DNA. These DNA sequences are compiled and analyzed for each sample to find out which are more similar to each other, or in other words, the same species group together.
The Guelph project — sponsored primarily by the federal and Ontario governments — began in 2003 after Dr. Hebert published a scientific article outlining his ‘‘DNA barcoding’’ method, originally developed as a tool for the identification and discovery of species diversity. (The technique was dubbed DNA barcoding, because it is like the universal product code – the black-and-white striped labels scanned by cashiers at stores. Last year, the U.S. Food and Drug Administration adopted this technique as a regulatory method for identifying food products.)
Dr. Hebert — who became fascinated with collecting insects as a child, and went on to a PhD at Cambridge — was primarily focused on butterflies and moths, so he set up working groups of scientists to cover the other animals and plants. To spread the net even wider, he then concentrated on establishing barcoding hubs on different continents that were connected in cyberspace, with Guelph as the epicentre.
Among the many researchers worldwide using the DNA facility is Scott Miller, deputy under-secretary for collections and interdisciplinary support at the Smithsonian Institution in Washington, D.C., who sends his insect samples from Papua New Guinea to the Guelph lab. Even though the Smithsonian has DNA capabilities, Dr. Hebert’s lab is the world’s leader in barcoding.
Dr. Miller said the barcode project has not only helped his research but has also been important in many non-scientific ways, such as air safety, by identifying the species most commonly involved in “bird strikes with airplanes.”
“It’s a virtuous and beautiful science” to bridge the gap between pure and applied science, said Dr. Hebert.
The DNA barcoding project has spread to 28 countries around the world, all contributing to the Guelph-based database. But there is still much work to be done because the 240,000 species in the genetic reference library account for only about 12% of the 2 million known species, or just under 0.25% of the upper estimate of 100 million species. It is currently populated by many of the large, better-known and easily found species, such as fish, birds and mammals.
The challenge over the next decade is barcoding the small, poorly known and difficult-to-find species, such as insects, fungi and single-celled organisms, Dr. Hebert said.
Dr. Hebert — who confesses to spending little time in the lab now that the project is spreading beyond academia — predicts that “we will see coverage for 1.5 million species by 2020.”
It took about 200 years for biologists to document that number of species the old way.